Journal: Cellular Oncology

Article Title: Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistance

doi: 10.1007/s13402-026-01198-2

Figure Lengend Snippet: Early-stage OSCC samples with lymph node metastasis showing elevated matrix stiffness and SGs level. ( a ) Masson staining showed fibrosis degree between pN0 (cancer tissue of pN0) and pN+ (cancer tissue of pN+) group. ( b ) Comparisons of the expression of G3BP1 between pN0 and pN+ group. ( c ) Comparisons of the expression of TIA1 between pN0 and pN+ group. ( d ) Correlation analysis showing the relation between SGs level and percentage collagen fiber content (%CFC) in early-stage OSCC. ( e ) Representative immunofluorescence staining of G3BP1 between pN0 and pN+ group. *p value<0.05, **p value<0.01, ***p value <0.001, ****p value<0.0001. The imagescales of ( a ), ( b ) and ( c ) represent 100μm. The imagescales of ( e ) represent 20μm

Article Snippet: OSCC cell lines Cal27 (ATCC Cat# CRL-2095, RRID: CVCL_1107) was purchased from American Type Culture Collection (ATCC).

Techniques: Staining, Expressing, Immunofluorescence

Journal: Cellular Oncology

Article Title: Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistance

doi: 10.1007/s13402-026-01198-2

Figure Lengend Snippet: Elevated matrix stiffness increased early-stage OSCC lymph node metastasis and SGs level in vivo. ( a ) Schematic of the in vivo experiment. ( b ) Masson staining showed fibrosis degree between NC (Normal Control) and FI (Fibrosis) group. ( c ) Comparisons of the expression of G3BP1 between NC and FI group. ( d ) Comparisons of the expression of TIA1 between NC and FI group. ( e ) HE and immunohistochemistry of CK5/6 showed the lymph nodes condition with negative metastatic focus and positive metastatic focus. The bar chart showed the condition of mice with lymph-nodes metastasis in each group. ( f ) Representative immunofluorescence staining of G3BP1 between NC group and FI group. *p value<0.05, **p value<0.01, ***p value <0.001, ****p value<0.0001. The imagescales of ( b ), ( c ) and ( d ) represent 100μm. The imagescales of ( e ) represent 200μm. The imagescales of ( f ) represent 20μm

Article Snippet: OSCC cell lines Cal27 (ATCC Cat# CRL-2095, RRID: CVCL_1107) was purchased from American Type Culture Collection (ATCC).

Techniques: In Vivo, Staining, Control, Expressing, Immunohistochemistry, Immunofluorescence

Journal: Cellular Oncology

Article Title: Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistance

doi: 10.1007/s13402-026-01198-2

Figure Lengend Snippet: Mechanical stress triggered SGs assembly in vitro. ( a ) Immunofluorescence showed SGs production of Cal27 stimulating by mechanical force. ( b ) Immunofluorescence showed SGs production of SAS stimulating by mechanical force. ( c ) Western bolt showed mechanical force up-regulated SGs level with a strength-dependent manner in Cal27 (upper) and SAS (lower). ( d ) Western bolt showed mechanical force up-regulated SGs level with a time-dependent manner in Cal27 (upper) and SAS (lower). ( e ) Representative electron microscopy image of SAS after stimulating by mechanical force. Red boxes mark the ER; red dotted lines indicate SG. The imagescales of ( b ) and ( c ) represent 10μm. The imagescales on the left of ( e ) represent 1μm, on the right represent 500nm

Article Snippet: OSCC cell lines Cal27 (ATCC Cat# CRL-2095, RRID: CVCL_1107) was purchased from American Type Culture Collection (ATCC).

Techniques: In Vitro, Immunofluorescence, Western Blot, Electron Microscopy

Journal: Cellular Oncology

Article Title: Stiffness-related stress granules promote the metastasis of early-stage oral squamous cell carcinoma via anoikis resistance

doi: 10.1007/s13402-026-01198-2

Figure Lengend Snippet: In vitro assays showed matrix stiffness facilitated anoikis resistance of OSCC through SGs formation in SAS. ( a ) Transwell assay showed the ability of migration and invasion for 48h after stimulating by mechanical force with or without G3la. ( b , c ) Flow cytometry showed the percentage of G0 cells after stimulating by mechanical force with or without G3la. ( d ) Analysis of resistance to anoikis stimulating by mechanical force with or without G3la. ( e ) Immunofluorescence showed morphological changes of SAS stimulating by mechanical force with or without G3la. ( f ) Representative images of the lung condition with negative metastatic focus and positive metastatic focus. The bar chart showed the condition of mice with lung metastasis in each group. *p value<0.05, **p value<0.01, ***p value <0.001, ****p value<0.0001. The imagescales of ( a ) represent 100μm. The imagescales of ( e ) represent 50μm. The imagescales on the left of ( f ) represent 1mm, on the right represent 100μm

Article Snippet: OSCC cell lines Cal27 (ATCC Cat# CRL-2095, RRID: CVCL_1107) was purchased from American Type Culture Collection (ATCC).

Techniques: In Vitro, Transwell Assay, Migration, Flow Cytometry, Immunofluorescence